ADCY5 ELISA 试剂盒
This 人 ADCY5 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 ADCY5.
Quick Overview for ADCY5 ELISA 试剂盒 (ABIN2042779)
抗原
See all ADCY5 products
ADCY5
(Adenylate Cyclase 5 (ADCY5))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.31-20 ng/mL
应用范围
ELISA
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最低检测浓度
- 0.31 ng/mL
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品牌
- BioAssay™
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灵敏度
- < 0.124 ng/mL
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Adenylate Cyclase 5 (ADCY5) ELISA Kit
ADCY5 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Adenylate Cyclase 5 (ADCY5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 5 (ADCY5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 5 (ADCY5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 5 (ADCY5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Adenylate Cyclase 5 (ADCY5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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